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B95-8(EBV陽性棉頂狨猴淋巴細胞)

CBP61105

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I. General information 
Synonyms: B95-8
Background: established from peripheral blood lymphocytes of a cotton-top tamarin (cotton-top marmoset) monkey (Saguinus oedipus); cells were described in the literature to release high titers of Epstein-Barr virus (EBV) providing a source for transformation of B cells and establishment of continuous cell lines
Species: monkey - cotton-top tamarin (Saguinus oedipus)
Tissue: N/A 
Disease: lymphocytes
Gender: N/A 
Morphology: single, round to oval cells or clustered in small clumps in suspension and partially adherent; ca. 10% adhere, but trypsin is not necessary to detach the cells; 
Growth Mode: N/A 
Doubling Time: ca. 41 hours
DNA Profile: N/A
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Culture Medium:

RPMI-1640+10%FBS

B95-8完全培養(yǎng)基,# CBP61105M
We strongly suggest to purchase the complete medium from us.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: N/A 
Fingerprint: unique Hinf I-(gtg)5 DNA profile
Species: Sanguinus oedipus origin confirmed by COI DNA barcoding
Cytogenetics: near-diploid simian karyotype with 4% polyploidy - 44(42-47)<2n> - chromosome rearrangements present
Virus   ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV -
Comments: For more information, please contact us (4008-750-250).

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