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ACE2 over-expression/A549 cell line

CBPC0003

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I. Background
Human Angiotensin converting enzyme 2 (ACE2), also known as ACEH, is an integral membrane protein found on the surface of cells in the lungs, arteries, heart, kidney, and intestines.
 
II. Description

ACE2 serves as the entry point into cells for some coronaviruses, including the two strains that caused outbreaks of Severe acute respiratory syndrome (SARS-CoV) and coronavirus disease 2019 (COVID-19) (SARS-CoV-2).

 
III. Introduction
Host Cell: A549
Expressed gene: ACE2
Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Synonym(s): Angiotensin converting enzyme 2, ACEH, SARS-CoV, Coronavirus, COVID-19, SARS-CoV-2, ACE2 cells, ACE2 cell assay, covid19
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:

F12K+10%FBS+2ug/ml puromycin

Application(s): Antibody Neutralizing Assay with 2019-nCov-S Pseudovirus
Storage: Liquid nitrogen
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Lung
Disease: Carcinoma
Morphology: Epithelial cells, growing adherently as monolayer
Growth Properties: Adherent
 
Ⅴ. Representative Data
 

Figure 1. Recombinant A549 cell constitutively expressing full length human ACE2.

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